世界*實驗材料供應商 Dianova 正式授權上海起發(fā)為其中國代理， Dianova 在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務,上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務，簽約 Dianova 就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務,您的滿意將是我們zui大的收獲

 Dianova 中國代理, Dianova 上海代理， Dianova 北京代理，Dianova 廣東代理， Dianova 江蘇代理Dianova 湖北代理,Dianova 天津,Dianova 黑龍江代理,Dianova 內(nèi)蒙古代理,Dianova 吉林代理,Dianova 福建代理, Dianova 江蘇代理, Dianova 浙江代理, Dianova 四川代理,

Dianova公司為德國的病理免疫組化產(chǎn)品供應商，具備的抗體制備工藝及嚴格的質(zhì)控系統(tǒng)，產(chǎn)品在上享有良好的聲譽。

克隆號為H09的IDH1（isocitrate dehydrogenase 1, 異檸檬酸鹽脫氫酶1）鼠單克隆抗體為Dianova公司近期推出的產(chǎn)品之一，此抗體可特異性的檢測腦組織腫瘤中IDH1基因的R132H點突變。IDH1基因的第132密碼子的雜合突變是II 和III級神經(jīng)膠質(zhì)瘤（根據(jù)WHO的腫瘤學分類）的頻發(fā)事件。在大約70％的星形膠質(zhì)細胞瘤和少突神經(jīng)膠質(zhì)細胞瘤可發(fā)現(xiàn)IDH1基因的R132H點突變。

IDH1基因R132H突變在部分腦腫瘤中的頻發(fā)現(xiàn)象使克隆號為H09的IDH1抗體可高靈敏性和高特異性的用于此類腫瘤的鑒別診斷中：如從原發(fā)性膠質(zhì)母細胞瘤中區(qū)分出間變性星形細胞瘤、從毛細胞型星形細胞瘤或室管膜（細胞）瘤中區(qū)分出彌漫性星形細胞瘤（WHO分類II級），也可以用于具有IDH1突變的腫瘤細胞浸潤前沿的與反應性神經(jīng)膠質(zhì)增多癥的區(qū)分。因此，由于其高靈敏性和高特異性，此抗體在腦部腫瘤的分類分級中以及在檢測單一或少量浸潤腫瘤細胞時是非常有用的。

DIA-303 – Anti-CD3 e (Ms) aus Ratte (Klon: HH3E) für Paraffingewebe aus Maus – 500 µl

Art.-Nr.                        DIA-303

Spezifität                      CD3 e

Spezies-Reaktivität             Maus

Wirtsspezies                     Ratte

Isotyp                           IgG1/k

Klon                             HH3E

Klonalität (Mono-/Polyklonal)     monoklonal

Anwendung                        Immunhistochemie (IHC)

                                 Immunhistochemie (Paraffingewebe)
                                 Gefrierschnitte (IHC-F)
                                 Western Blot

Konjugation                       unkonjugiert

Verdünnung                        Histo-/Zytochemie 1:100

Format                            Lyophilisat

                                  gereinigter Antikörper (aus Kulturüberstand)

Produktlinie / Thema               CD-Marker

                                   Immunhistochemie

Zweckbestimmung                     nur für Forschungszwecke

Suchcode                             DIA303M

                                     DIA303

Hersler / Marke                   dianova

Reactivity:

Antibody clone HH3E has been validated specifically for the detection of murine CD3 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). It detects a conserved epitope on the CD3 epsilon chain in a broad variety of species. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.
Cluster of differentiation 3 (CD3) is composed of four distinct polypeptide chains CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta, that form a multimeric protein complex. The CD3 complex associates non-covalently with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal trans-duction molecules. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into in-tracellular signals that elicit a change in the cell from a dormant to an activated state. During T cell maturation the expression of CD3 migrates from the cytoplasm of pro-thymocytes to the cell-membrane of thymocytes. The specific appearance at all stages of T cell development make CD3 an ideal marker for normal T cells and T cell neoplasms (lymphomas, leukemias). Moreover, CD3 is a usefull immunohistochemical marker for T cells in tissue sections.
In a clinical setting in humans, CD3 serves as an important T cell marker for the classification of malignant lymphomas and leu-kemias. It can also be used to detect T cells in coeliac disease, lymphocytic and collagenous colitis. An anti-CD3 epsilon anti-body (Okt3) has been clinically approved for the induction of immunosuppression in organ transplantation. In animal studies anti-CD3 antibodies can induce tolerance to allografts.

Immunohistochemistry of mouse CD3e (TCRE) in formalin-fixed paraffin-embedded tissue sections

The monoclonal antibody clone HH3E specifically stains mouse tissue sections by IHC-FFPE: Lymph nodes (A,B), spleen (C) and kidney (D).

All sections were stained by an indirect alkaline phosphatase method according to standard procedures with antigen retrieval by high-temperature heating in citrate buffer and counterstaining with Haematoxylin.

A

B

C

D

Related References:

1. Leon F. Flow cytometry of intestinal intraepithelial lymphocytes in celiac disease. Journal of Immunological Methods 363: 177-186, 2011
2. Smith-Garvin JE et al.. T cell activation. Annu Rev Immunol. 27:591-619, 2009
3. Sapp H et al.The terminal ileum is affected in patients with lymphocytic or collagenous colitis. Am J Surg Pathol. 26(11):1484-1492, 2002
4. Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clon-ality by polymerase chain reaction. Vet Immunol Immunopathol. 69:145-164, 1999
5. Mosnier et al. Lymphocytic and collagenous colitis: an immunohistochemical study. Am J Gastroenterol. 91(4):709-713, 1996
6. Chetty R, Gatter K. CD3: Structure, function, and role of immunostaining in clinical practice. The Journal of Pathology 173(4): 303–307, 1994
7. Salvadori S et al. Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors. J Immunol. 153(11):5176-5182, 1994
8. Nicolls MR et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation 55(3):459-468, 1993

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